Collagen fibrils were reconstituted in vitro from type I collagen solution. The reconstituted collagen fibrils and chondroitin sulfate B were deposited alternately. The adsorbed weight of the collagen fibrils and chondroitin sulfate B was measured using a quartz crystal microbalance (QCM) and their microstructure was observed using an atomic force microscope (AFM). https://www.foldlyers.shop/product-category/megaphones/